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To produce RMLA images showing actin filaments, mClover3 and Lifeact-mRuby3 images were background subtracted by using Fiji’s “Image Calculator” function to subtract the background image acquired away from cells from the images. Each mClover3 and Lifeact-mRuby3 frame was then aligned using...
DNA double-strand breaks (DSBs) are a severe form of DNA damage, but eukaryotes have evolved a number of repair pathways16. DSB repair pathway choice, i.e. the cellular decision of which mechanism to utilize for DSB repair, is controlled at the step of DNA end resection17. Resection is ...
(AAI) was calculated using the All-vs-All AAI matrix calculator tools developed at the Kostas lab [52] (Supplementary Table S1). The isoelectric point of Woeasearchaeota proteins were calculated using custom Python 3.10 and the Bio.SeqUtils package. Amino acid ratios of all predicted proteins...
(AAI) was calculated using the All-vs-All AAI matrix calculator tools developed at the Kostas lab [52] (Supplementary Table S1). The isoelectric point of Woeasearchaeota proteins were calculated using custom Python 3.10 and the Bio.SeqUtils package. Amino acid ratios of all predicted proteins...
Keisan Online Calculator Casio Computer Co., Ltd https://keisan.casio.com/ Samtools 1.9 Li et al.120 http://www.htslib.org/ Resource availability Lead contact Further information and requests for resources and reagents should be directed to and will be fulfilled by the lead contact, Jose C...
For amino acid composition analysis, the web application Prot Pi Protein Tool https://www.protpi.ch/Calculator/ProteinTool was used. Disordered proteins were defined by the presence of a 50 residues fragment whose IUPRED median score was at least of 0.55 and that was not found in Pfam, a ...
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First, a myofibrillar mask with myofibrillar pixels assigned a value of 1.0 and background pixels assigned as null (NaN or ignored in all mathematical analyses) values were created by dividing the myofibrillar image by itself using the Image Calculator tool while choosing 32-bit (float) result. ...