The sensitivity of the DENV RT-PCR method ranges from 28.8 to 99%. NS1 antigen is used as an initial diagnostic option in primary health care because it has a high specificity value (100%). Researchers want to analyze the positivity in non-DVI samples that have been confirm...
infection confirmed dengue infection • Virus isolation • Genome detection • Antigen detection • IgM or IgG seroconversion Probable dengue infection • IgM positive • Elevated IgG titre (that is, 1,280 or greater by haemagglutination inhibition test) Reverse transcriptase PCR (RT-PCR)....
NS1 detection appeared to be better than RT-PCR. For example, DENV NS1 antigen detection in travelers upon arrival at airports in Taiwan was instrumental in the detection of 19 RT-PCR-negative travelers who would have been labeled DENV negative. Two of them turned out to be IgM positive on...
In addition to the reduction in viral protein expression after RPL18 silencing, one log reduction in the levels of viral RNA was also distinguished by qRT-PCR in cells transfected with 150 nM of RPL18 protein siRNA and harvested at 48 hpi compared with the amount of viral RNA present in ce...
component of the vaccine [34]. Moreover, differential rates of viral replication of each vaccine component were observed by RT-PCR in dengue-naïve individuals after receiving one vaccine dose: DENV-4 (44%), DENV-3 (12%), DENV-1 (7%), and DENV-2 (0%), which may pose a risk for...
At the end of the last century, India has faced resurgence of many infectious diseases, of which dengue is one of the most important in terms of morbidity
Empty Cell Empty Cell Empty CellRT-PCRaNS1aIgMa Diagnostic test NS1 Sensitivity 0.420 (0.311–0.538) Specificity 0.889 (0.838–0.926) PLR 3.80 (2.35–6.16) NLR 0.65 (0.53–0.80) DOR 5.83 (3.04–11.19) IgM Sensitivity 0.130 (0.070–0.229) 0.412 (0.287–0.548) Specificity 0.904 (0.85...
In early phase of dengue NS1 and PCR were found to be better tests for diagnosis and later IgM is better. The IgG/IgM ratio of ≥ 1.10 had a sensitivity of 100%, specificity of 97.4% and accuracy of 67.5% in differentiating secondary from primary dengue. Early on in the clinical ...
Reverse transcription (RT)-PCR was performed for the amplification of the full coding region of each DENV isolate using a QIAGEN OneStep RT-PCR Kit (QIAGEN, CA, USA). The primers were described previously [22,23], and their sequences are shown in Supplementary Table1. The thermal cycling ...
diagnosis is established by detecting virus in clinical samples, serologic responses usingIgM antibodycapture enzyme-linked immunosorbent assay (MAC-ELISA), or viralnucleic acidsin serum or CSF using real time, reverse transcriptionPCR. At present, treatment consists of fluid replacement, supportive care...