1.to deprive (something) of its natural character, properties, etc. 2.to render (various alcohols) undrinkable by adding an unwholesome substance. 3.to treat (a protein or the like) by chemical or physical means so as to alter its original state. ...
rTHS prevents protein aggregation and maintains/restores enzyme activity in the direct presence of co-solvents. Given the unfolding pathways of the enzyme, we can predict how well rTHS inhibits enzyme deactivation and, when appropriate, can modify the unfolding environment to assist rTHS function. ...
An increase in the denaturant concentration in the denatured system can significantly enhance the inactivation and unfolding of APE1547. The enzyme can be completely inactivated with a urea concentration of 2. 7 mol/L or a GdnHCl concentration of 7. 5 mol/L. The fluorescence emission maximum ...