The delta-delta Ct method, also known as the 2–∆∆Ct method, is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction (also known as qPCR). The method was devised by Kenneth Livak and Thomas Schmittg...
Molecular solubility is among the key properties that determine the clinical performance of a drug candidate because poor molecular solubility often indica
Calif., USA). Concentrations of primers were 900 nM for each, and that of the probe was 200 nM. The total reaction volume of PCR was 10 μL, including 5 μL of 2× LightCycler 480 Probes Master (ready to use hot start PCR mix), composed of dNTP mix (with dUTP instead ...
The second round reaction was carried out with 1× buffer, 2 mM MgCl2, 0.2 mM dNTPs (Promega), using the primers HDV-E and HDV-A at 0.5 µM [22], and Taq DNA polymerase (Thermo Scientific) 1.25. There were cycles of 95 °C for 2 min, followed by 35 cycles of 95 °C for ...