What is the delta-delta Ct method? The delta-delta Ct method, also known as the 2–∆∆Ctmethod, is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction (also known as qPCR). The method was devised...
(0.00–0.20) respectively. Both the models show that soil fertility is respectively higher near the Hooghly River bank. In many cases, the results obtained from FAHP and FL are quite similar but huge dissimilarity has been noticed in grid numbers G2, G3, G4, F1, and F2. Since the ...
Calif., USA). Concentrations of primers were 900 nM for each, and that of the probe was 200 nM. The total reaction volume of PCR was 10 μL, including 5 μL of 2× LightCycler 480 Probes Master (ready to use hot start PCR mix), composed of dNTP mix (with dUTP instead ...