The bilateral guide cannula was held in place using dental cement (Dentalon plus, Heraeus Kulzer GmbH, Hanau, Germany) and two stainless-steel screws. The placement was set at 1 mm above the target injection site and the guide cannula was sealed with a dummy of stainless- steel wire with ...
All electrodes were attached to a microconnector and fixed to the skull with dental cement, as described previously (Qu et al, 2008). Cortical EEG and EMG signals were amplified and filtered and then recorded by using SLEEPSIGN (Kissei Comtec). After EEG/EMG implantation, mice were housed ...