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The purified deionized water was prepared by the Milli-Q plus system (Millipore Co., Billerica, MA, USA). 2.2. Preparation of CaNP/DOX/Cyt c Firstly, methoxy poly(ethylene glylcol)-block-poly(l-glutamic acid) (mPEG-b-PGA) was synthesized based on our previous work [35]. Then, mPEG...
The protein was then transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk or 5% bovine serum albumin (BSA) and incubated with specific primary antibodies against mouse ACE2 (1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, ...
Linda C Billerica25 contributions 0 Good casual dining Sep 2018 • couples It was our anniversary and we wanted to go to a casual restaurant. We’ve never been to chili’s before. I got the mango chicken, which was very tasty. My usband got a burger and fries. He said i...
The protein was then transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk or 5% bovine serum albumin (BSA) and incubated with specific primary antibodies against mouse ACE2 (1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, ...
The values of TEER were determined by measuring the potential difference between the two sides of the cell monolayer using a (Millicell ERS-2, Millipore Corporation, Billerica, MA) connected to a pair of chopstick electrodes. On the day of experiments, the cells were washed twice with phosphate...
Gonadorelin-coupled PEG-DSPE micelles were then con- centrated in a Centriprep® Centrifugal filter, using a size cutoff of 3000 Da (Millipore, Billerica, MA). The amounts of conjugated and free gonadorelin were indirectly measured with a bicinchonic acid protein assay using pure bovine serum...
StreetPretzel North Billerica323 contributions 1 Great Meal at Birchtree Bread Jul 2021 • none My family greatly enjoyed our first post-Covid visit to Birchtree. The experience was actually better than before. I like the being able to sit first, order on the app, and h...
Gonadorelin-coupled PEG-DSPE micelles were then con- centrated in a Centriprep® Centrifugal filter, using a size cutoff of 3000 Da (Millipore, Billerica, MA). The amounts of conjugated and free gonadorelin were indirectly measured with a bicinchonic acid protein assay using pure bovine serum...