Nic-SMEDDS (100 µL) was pre-diluted with deionized water (1:2 v/v%), equivalent to about 5.6 mg niclosamide, and added to the dialysis tube (D-TubeTM Dialyzer, MWCO 6–8 kDa, Novagen®, Merck KGaA, Billerica, MA), which was then placed in Dissolution Medium (0.2 M phosphate ...
The protein was then transferred to nitrocellulose membranes (Millipore, Billerica, MA, USA). Membranes were blocked with 5% skim milk or 5% bovine serum albumin (BSA) and incubated with specific primary antibodies against mouse ACE2 (1:200, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, ...
The final product was confirmed by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS; Ultraflex b, Bruker Dal-tonics, Billerica, MA, USA). The synthetic route and mass spectra of C-siRNA (sense strand: CPP-S-S-5′-GUGAUGAAGUACAUCCAUUdTdT-3’; ...
Subsequently, sucrose solution (20–25% sucrose) containing the Cy5.5-labeled protein nanoparticles or RFP was fractionated and tion then exchanged to PBS (2.7 mM KCl, 137 mM NaCl, (Amicon Ultra 100K, Millipore, Billerica, MA). 2 mM KH2PO4, 10 mM Na2HPO4, pH 7.4) by ultra...
HPEKp cells were maintained in CnT-57 medium and seeded (200,000 cells in 400 μl) in 12-mm inserts lined with a 0.4 μm porous membrane (Millipore, Billerica, MA). The inserts were maintained in a 6-cm plate containing 10.5 ml CnT-57 medium. After 2–3 days, the CnT-57 medium...
Purified product was characterized using a Bruker Autoflex III Smartbeam Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometer (MALDI-TOF-MS, Billerica, MA). Cell culture H929, MM1S, RPMI, and IM9 were obtained from American Type Culture Collection (Rockville, MD) and ...
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The purified deionized water was prepared by the Milli-Q plus system (Millipore Co., Billerica, MA, USA). 2.2. Preparation of CaNP/DOX/Cyt c Firstly, methoxy poly(ethylene glylcol)-block-poly(l-glutamic acid) (mPEG-b-PGA) was synthesized based on our previous work [35]. Then, mPEG...
The values of TEER were determined by measuring the potential difference between the two sides of the cell monolayer using a (Millicell ERS-2, Millipore Corporation, Billerica, MA) connected to a pair of chopstick electrodes. On the day of experiments, the cells were washed twice with phosphate...
Samples were drawn into a pre-warmed glass syringe, filtered through a syringe filter (Mineral oil: Millex FG-13, Millipore, Billerica, Mass., USA, and buffer: nylon filter, Gelman, East Hills, N.Y., USA), and measured with respect to volume and diluted with an appropriate volume of ...