RNA Isolation and Northern Blots Total RNA was extracted by utilizing the guanidine thiocyanate-phenol method as described by Chomczynski and Sacchi (19). For Northern blots, 20 μg of total RNA was separated in a 1% agarose-formaldehyde gel, transferred to Hybond-N+ nylon membranes (Amersham...
Plasmids recovered from 4 independent colonies obtained from this well have a cDNA insert of 2.6 kb and can be transcribed to produce an RNA that encodes a 74 kDa protein in reticulocyte lysates, which binds to Xenopus chromosomes. We named the plasmid encoding this protein pSP70-1. Sequence ...
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