The size of the implant also plays a role in immune response. Larger implants produce a proportionally higher magnitude of foreign body reaction and fibrosis. For polymers that are susceptible to autocatalysis, in addition to being dependent on flow conditions at the implantation site, there is a...
Oxidation: An important enzyme reaction in fungal degradation of cellulose. FEBS Lett. 1974; 49 :282–285. doi: 10.1016/0014-5793(74)80531-4. [ Cross Ref ]Eriksson, K.E.; Pettersson, B.; Westermark, U. Oxidation: An important enzyme reaction in fungal degradation of cellulose. FEBS Lett...
Cellulase activity was evaluated according to the method of Miller45as described by Anandet al.46. Briefly, 500 μL 1% carboxymethyl cellulose (CMC) as substrate was added in 1 mL PBS (0.1 M, pH 6.8) with 500 μL sample and incubated at 37 °C for 1 h. Reaction was stopped by a...
In the canonical ubiquitination pathway, ubiquitin is conjugated to target proteins by an E1-E2-E3 enzymatic cascade (Fig.1). As the E3 ligase is responsible for recognizing substrates and its family number greatly exceeds E1 and E2, the UPS-based TPD strategies utilize E3 ligases as targeting ...
Each enzyme has a unique role in the proteolytic process of degradation (Figure 1). Ubiquitin activation: The E1 ubiquitin-activating enzyme activates Ub in an ATP-dependent two-step reaction. Ubiquitin conjugation: The E2 ubiquitin...
nucleophile to attack the carbonyl carbon (C) of the substrate to afford an acyl-enzyme intermediate. Then, the R2group is removed from the polymer in an alcohol form due to an elimination reaction. The acyl-enzyme intermediate is then attacked by a water molecule (H2O) to afford the acid...
the expression of TaPIR1−4A (Puccinia striiformis-induced RING-finger protein 1), a member of the E3 ubiquitin-protein ligase family, was induced at 24 h post-inoculation (hpi), as described in the quantitative reverse transcription polymerase chain reaction (qRT-PCR) results (Supplementary ...
Enzyme mediated decolorization is another newer alternative, where the enzyme can specifically react with organic pollutants by transforming them into low molecular weight products[23]. The main advantage of using enzymes in degrading dye solutions is that they have higher reaction rates and can also ...
Design of an Amphiphilic Polymer for Nanoparticle Coating and Functionalization. Small 2008, 4, 334–341. [CrossRef] 29. Liang, L.Y.; Astruc, D. The copper(I)-catalyzed alkyne-azide cycloaddition (CuAAC) "click" reaction and its applications. An overview. Coord. Chem. Rev. 2011, 255,...
The enzymatic hydrolysis was carried out in 0.1 M sodium phosphate buffer using the optimum pH for each enzyme, using 20 (with 50 mg substrate) or 50 mL (with 100 mg substrate) as reaction volume. The dosage of each enzyme was 0.1 U·mg−1 substrate (with 1 unit defined as the ...