Briefly, we integrated doxycycline-inducible CBE or ABE NGN base editors into the CLYBL safe-harbor locus using CRISPR–Cas9 and homology-directed repair, and used blasticidin and mApple (fluorescence-activated cell sorting (FACS)) to select for base editing cell populations. We used BE-FLARE87 ...
Briefly, we integrated doxycycline-inducible CBE or ABE NGN base editors into the CLYBL safe-harbor locus using CRISPR–Cas9 and homology-directed repair, and used blasticidin and mApple (fluorescence-activated cell sorting (FACS)) to select for base editing cell populations. We used BE-FLARE87 ...
H.S. analysed bulk and single cell RNA-seq data, and performed FACS sorting and ATAC–seq. Zhaoyuan Liu and F.G. provided expert advice about scRNA-seq data analysis. M.N., L.G.N. and Y.Y. provided expert advice in ATAC–seq data analysis. X.Z. and Y.Z. performed RNAScope ...
The most simple deterministic algorithm is thisrandom number generator. For instance if you are sorting elements that are strictly ordered(no equal elements) the output is well defined and so the algorithm is deterministic. In fact most of the computer algorithms are deterministic. ...
A. L. Burlingame and Robert Chalkley for supporting the use of Protein Prospector and David Vong for help in data sorting. Supplementary Material Download: Download zip file (30MB)REFERENCES 1 D.P. Ryan, J.M. Matthews Protein-protein interactions in human disease Curr. Opin. Struct. Biol, ...
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the role of Rab17 in PD progression is not fully understood. At the phosphoprotein level, only Rab12 (p < 0.005) was significantly upregulated in LRRK2 PD (Supplementary Fig.12a). Rab12 is an LRRK2 endogenous substrate that plays a role in endosomal lysosome sorting, degradation, and...
If you’re using a sort function to arrange different string lists in alpha order, the browser might only be able to reference the sort order for the current locale it’s displaying. Make sure you handle the sorting on the server instead. Otherwise, you’ll end up using English alphabetical...
(e.g. different locality definitions / aspects that can be declared as preferred or needed by a requestor). but eventually i realized that Preference type will be needed for sorting rather than comparing; it is nevertheless left, because all comparator signatures now include optional parameter so...
Based on this preliminary clini- cal finding, we hypothesized that CD105+Nestin+ cells outside the tumor front represent a subset of GSCs and using fresh tissue from tumor and peri-tumor regions, we purified the CD105+ cells by fluorescence-activated cell sorting (FACS) and ...