The introduction of protease inhibitors (PIs) as part of the highly active antiretroviral therapy (HAART) have led to a dramatic reduction in morbidity and mortality rates in HIV-1–infected patients1. PIs have high intrinsic antiviral activity and are among the most potent antiretroviral drugs (A...
In brief, 1 × 106 cells were injected into 10–12 week old female NOD scid gamma mice through the lateral tail vein. Mice were immediately sacrificed post-injection. The lungs of the mice were insufflated with a 50% medium/agarose solution. The lungs were then resected and placed in ice...
Male reproductive system ageing is closely associated with deficiency in testosterone production due to loss of functional Leydig cells, which are differentiated from stem Leydig cells (SLCs). However, the relationship between SLC differentiation and age
Briefly, 5 mL of peripheral blood were lysed with non-fixing ammonium chloride-based lysing reagent (FACSLysing Solution®, BD) for 10 min. A total of 100,000 cells per tube were incubated 1 or 2 times (depending on the labelling) for 20 min at room temperature with pre-titrated ...
Briefly, 5 mL of peripheral blood were lysed with non-fixing ammonium chloride-based lysing reagent (FACSLysing Solution®, BD) for 10 min. A total of 100,000 cells per tube were incubated 1 or 2 times (depending on the labelling) for 20 min at room temperature with pre-titrated ...