The mass spectrometer was set to acquire data in positive-ion mode using selected reaction monitoring acquisition. Three transitions were acquired for each target analyte, the cycle time set to 0.8 s, Q1 resolution to 0.7 full-width at half-maximum, Q2 resolution at 1.2 full-width at half...
reduction with 0.5 M dithiothreitol at 56 °C for 20 min and alkylation with 0.55 M iodoacetamide at room temperature for 15 min, in the dark. One μl of a 1% w/v solution of ProteaseMax Surfactant Trypsin Enhancer (Promega) and 1 μg...
Genome-wide expression profiling identified five biological BC subtypes36, namely luminal A/B, HER2-enriched, basal-like and normal breast-like, which differ by hormonal receptor status, proliferation, genomic instability, mutational signatures, treatment response and prognosis37,38,39,40,41. It has...
reaction rate DEFINE_SR_RATE User-Defined Function Hooks volume reaction rate DEFINE_VR_RATE User-Defined Function Hooks turbulent premixed DEFINE_TURB_PREM User-Defined Function source IX_SOURCE Hooks scalar flux function DEFINE_UDS_FLUX User-Defined Scalars scalar unsteady DEFINE_UDS_UNSTE User-...
The possible reaction sites were restricted to C-terminal extremities and acidic side chains of Glu and Asp amino acids. Accuracy on mass measurements was 2 ppm for the precursor and 10 ppm for the fragment ions, to reduce to their maximum the false positives and to increase the reliability ...
In addition, the performance of CO2 reduction can be remarkably improved at a higher concentration due to the increased reaction kinetics and alleviated concentration overpotential. Attributing to the buffer effect of bicarbonate, the flow rate of the electrolyte exhibits leB influence on the electrode ...
Several methods have been developed for inferring single-cell trajectories (Trapnell et al., 2014); however, these usually rely on dimensionality reduction, which makes it hard to infer the regulatory process that controls the branching of various cell fates (Ding et al., 2018). To address ...
7. Early diagnosis paired with prompt and effective therapeutic interventions in case of infection risk may lead to better treatments targeting the causes of wounds, as well as reduction of wound infections. Clearly, there is an unmet need for improved diagnostic approaches in order to better ...
The reaction was quenched with hydroxylamine to a final concentration of 0.3% (v/v). The TMT-labeled samples were pooled at a 1:1:1:1:1:1:1:1 ratio across the 8 samples. The pooled sample was vacuum centrifuged to near dryness and subjected to C18 solid-phase extraction (Sep-Pak,...
The coupling reaction of 16 with 1,4-xylylenediamine produced 17. Reduction and removal of the benzoyl groups generated 6, while RCM, alkene reduction and benzoyl group removal established the glycophane 5. As outlined in Scheme 2, the synthesis of compounds 7 and 8 was achieved from alkyne ...