To test the effect of H2O2 on GAPDH activity, various concentrations of H2O2 were added to the reaction (0.05 μM PvGAPC1, 10 μM Na2HAsO4, 1.25 mM Ga-3-P, 2.5 mM NAD+ in the GAPDH reaction buffer) just before reading. Percentage GAPDH activity was calculated by normalizing against ...
vivo15,16. Interestingly, we found thatPseudomonas aeruginosamay mimic the formation of such HDPs, leading to generation of anti-inflammatory peptides and circumvention of host responses17. In a recent report, we also demonstrated a previously undisclosed role of larger TCPs of about 11 kDa, in...
cell differentiation, proliferation and growth, cancer invasion, and chemotherapy resistance. In humans, SLFN11 was reported to inhibit HIV protein synthesis by a codon-usage-based mechanism57and in non-human primates positive selection on the geneSlfn11has been reported58. In mouse...
The proteins in the different families cover a wide range of activities, on the level of glycan routing and transport, cell adhesion and growth regulation as well as host defense, to give a few examples (for further information, please see [35,36]). Of note, the binding is remarkably ...
In this mechanism, the bond dissociation enthalpy (BDE) is an important parameter in evaluating the antioxidant action [5]. The lower the BDE of the H-donating group in the potential antioxidant, the easier it will be for the reaction of free radical inactivation. The SET reaction is ...
The role of vitamin C in the human immune defense in particular is a widely researched field. However, the heterogeneity of study designs and the variability or even inconsistency of outcomes make it difficult to use these data as the basis for daily reference values. In the past, they were...