For qPCR SYBR-Green PCR Mastermix (BioRad) was used and the qPCR was run on a Biorad CFX connect machine using the Biorad CFX Maestro 1.0 software. Gene expression data were normalized to beta-actin and the data are presented as fold change. The primer sequences can be found in Suppl...
15,16,17. In particular, the reported expression of members of the galectin family, tissue lectins with β-galactoside binding capacity18,19, has stimulated the research of their functional implications in nervous system physiology. So
residual (no treatment) and the treated response for each residue. The off-diagonal values in VarC global maps present the pairwise functional covariance in response to the treatment for any two different residues. On-diagonal values are used to build a “functional response structure” to defin...
WIZ occupancy in wild-type cells is shown. (D) Overlap of RAD21 peaks in wild-type and Wizdel cells. For shared RAD21 peaks and ectopic RAD21 peaks, the overlap with functional elements in the genome is shown (CTCF sites, enhancers, promoters, other). (E) Average signal plots and ...
vittata gametophytes with a PDS-1000/HE system (Bio-Rad) using 1.3 mM tungsten M-20 microcarriers. Particle bombardment was performed with 1,100-psi rupture disks and a shooting distance of 3 cm. For each RNAi experiment, gametophytes were bombarded with the 35S::DsRed2 plasmid (pSAT) [...
A method of interest to meet the compromise between accuracy and speed requirements, MEC can be used, especially in design optimization software [7,8,11,17,19,20]. The computation of an MEC model can be done by two methods [21]. The first method is the node-based analysis that is ...
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BHI broth was brought to the desired pH then calcium chloride in MilliQ water was added to achieve the final concentrations. After addition of spores to the growth media suspension, images were captured with a BioRad XR Gel Documentation System. Preparation and growth of the bacteria for these...