15,16,17. In particular, the reported expression of members of the galectin family, tissue lectins with β-galactoside binding capacity18,19, has stimulated the research of their functional implications in nerv
Although carbohydrate- binding domains have no catalytic activity of their own they play important roles in substrate recognition and binding of other CAZymes [87, 88], they are also involved in the protection of fungal cell walls from deg- radation by host enzymes and prevention of host detec...
vittata gametophytes with a PDS-1000/HE system (Bio-Rad) using 1.3 mM tungsten M-20 microcarriers. Particle bombardment was performed with 1,100-psi rupture disks and a shooting distance of 3 cm. For each RNAi experiment, gametophytes were bombarded with the 35S::DsRed2 plasmid (pSAT) [...
WIZ occupancy in wild-type cells is shown. (D) Overlap of RAD21 peaks in wild-type and Wizdel cells. For shared RAD21 peaks and ectopic RAD21 peaks, the overlap with functional elements in the genome is shown (CTCF sites, enhancers, promoters, other). (E) Average signal plots and ...
The resin was washed with 1.8 mL water, then re-supended in 1 mL SDS buffer and 10 mM dithiothreitol (DTT) (Bio-Rad) and reduced at 70°C for 15 min. After removal of the SDS/DTT buffer, the slurry was alkylated using 1 mL SDS buffer and 7.4 mg iodoacetamide (Bio-Rad) for 30...
(aMmdiRinuSism,(mu0.mi2n5irmamdumimal. srCeaupdarirarealntsiteolpyna,);- dfirelaftateiuro,lnt5)0m; fUeilatPseRur,r(i5un0ngdUcuoPlnRadti(ioutinnodnpsuelrfaortrieovrnooulpunetdrionrnee)sv;soaltnuodtlieotrinapn);craeandadiruetsi,np0o.rt2a5sdpimeucsmi,fi.0e.dC25uinrmretmhnet....
A method of interest to meet the compromise between accuracy and speed requirements, MEC can be used, especially in design optimization software [7,8,11,17,19,20]. The computation of an MEC model can be done by two methods [21]. The first method is the node-based analysis that is ...