Following several rinses in dH2O, samples were dehydrated in a graded series of ethanol and embedded in Eponate 12 resin (Ted Pella, Inc.). Sections of 95 nm were cut with a Leica Ultracut UCT ultramicrotome (Leica Microsystems, Inc.), stained with uranyl acetate and lead citrate, and ...
Analysis of the Flow Conditions in a Dosing Pump with Regard to New Fuels Conveying new fuels with a high proportion of ethanol leads to an increased cavitation tendency inside a pump. This paper presents investigations of the fl... M Petzold,J Weber,E Dautry,... - International Fluid Powe...
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Step-by-Step Text Solution:1. Definition of Vapor Pressure: - Vapor pressure of a liquid is defined as the pressure exerted by the vapor of the liquid in a closed container when the liquid and vapor are in t
Drug resistance is a principal limitation to the long-term efficacy of cancer therapies. Cancer genome sequencing can retrospectively delineate the genetic basis of drug resistance, but this requires large numbers of post-treatment samples to nominate ca
A sprayable film-forming preparation, e.g. for hair grooming, consists of a solution in a mixture of low-boiling alcohol and aerosol propellant of a copolymer of 30-90% by weight of N-vinylpyrrolidone with 10-70% by weight of a vinyl ester. Alcohols mentioned are methanol, ethanol and ...
Relevant statistics are referred to in each of the associated methods sections. We did not use statistical methods to predetermine a sample size and patients were not randomly selected as patients were enrolled as they entered the clinic. We excluded samples that did not pass sample preparation QC...
Preparation of culture supernatants from P. aeruginosa for analysis of secreted proteins has been described (Viarre et al., 2009). Gel analysis was standardized so that the volume of supernatant equivalent to 2 OD600 units of culture was used for each sample. Protease activity was tested by spo...
Individual cell shape and integrity must precisely be orchestrated during morphogenesis. Here, we determine function of type II cadherins,Cdh6, Cdh8, andCdh11, whose expression combinatorially demarcates the mouse neural plate/tube. While CRISPR/Cas9-based single type II cadherin muta...
no binding to the outer base pairs, 1 to 15, for either end, due perhaps to the prevalence of truncated N-terminal species in the preparation of the protein [26] or to the imprecise folding of the C-terminus, a process which appears to have been avoided in our GFP-tagged version [31...