PBS: Phosphate-buffered saline PCSK9: Proprotein convertase subtilisin/kexin type 9 PD: Parkinson's disease PFA: Paraformaldehyde PIG: Amyloid-β plaque-induced genes PL: Pyramidal layer PMD: Post-mortem delay pMLKL: Phosphorylated mixed lineage kinase domain-like protein PR: Progesterone...
To evaluate the presence of mitochondria, CHL-1 cells were seeded and treated as above. Cells were incubated with 3.7% formaldehyde in PBS for 15 min, washed with cold PBS and permeabilized with 0.5% Triton X-100 in PBS for 10 min. After washing three times with PBS, cells were blo...
25、xResponsibility matrixScope statementProcess breakdown structure (PBS)Work breakdown structure (WBS)Work package2022-1-22编辑ppt321 The method used to collect information to use through all phases of the project life cycle is called c A. Responsibility matrixB. Organization breakdown structureC. ...
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MG63.3 cells were isolated and processed according to the Nuclei Isolation for Single Cell ATAC Sequencing Demonstrated Protocol (10X Genomics, CG000169) with the following modifications: 1% BSA in PBS was used for the washes, and cells were lysed for 3 min on ice. Sequencing libraries were...
. Pooled diets were concentrated using a Vivacell 250 Pressure Concentrator (Sartorius Mechatronics, UK) using a 5000 Da molecular weight cut-off (MWCO) polyethersulfone (PES) membrane. When the final volume had reached 5 ml it was removed and 1 ml of filtered sterilised PBS (phosphate-...
UNITY_PBS_USE_BRDF1 UNITY_PBS_USE_BRDF1 is set if Standard Shader BRDF1 should be used. UNITY_PBS_USE_BRDF2 UNITY_PBS_USE_BRDF2 is set if Standard Shader BRDF2 should be used. UNITY_PBS_USE_BRDF3 UNITY_PBS_USE_BRDF3 is set if Standard Shader BRDF3 should be used. UNITY_NO_FULL...
Local pDCs were depleted by subconjunctival injections of diphtheria toxin or PBS (sham) in BDCA-2-DTR Tg mice. dLN were excised and assessed for T cell phenotype by flow cytometry with CD45 (pan-leukocytes marker), CD3 (T cells), CD8, and CD4, and production of IL-6 and TGF-尾1...
urine. The mixture was incubated for 1 h by end-over-end rotation, and the supernatant was removed using a magnetic separator rack; the beads were washed with PBS, and the EVs were eluted by a 10 min incubation with 100 mM triethylamine (TEA, Millipore-Sigma). The eluted samples ...
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