Expression profiling libraries were sequenced on Illumina HiSeq 3000/4000 instruments in 50-base-pair-single-end mode and base calls provided by the Illumina Real-Time Analysis (RTA) software were subsequently converted into BAM format (Illumina2bam) before de-multiplexing (BamIndexDecoder) into ind...
Each experimental condition and replicate received a unique barcode (index) for multiplexing. The sequences of oligonucleotides used are available in Table S1. For each strain or experimental condition at least two biological replicates (and up to 8) were prepared, and from each of these ...