We chose to perform PRE experiments using a 10:3 molar ratio of 15N XcpHp to spin-labeled XcpJp variants. Although a 1:1 ratio would in theory have provided more quantitative results, for this protein pair, it would have led to signal loss as seen in the previously described titration. ...
Membrane traffic operates by vesicles that bud from precursor organelles and are transported to their target compartment where they dock and fuse. Targeting requires tethering factors recruited by small GTPases and phosphoinositides whereas fusion is car
N protein is among the most abundant SARS-CoV-2 proteins9. As ORFNis immediately upstream ofORF10inNsgRNA, translation ofNcould disrupt RNA structures driving internal initiation ofORF10, thereby facilitating re-initiation ofORF10in the 3′-UTR, as anNsgRNA-specific route forORF10translation41. ...