Among regions that overlap hypermethylated probes in CRC that become less accessible in polyps are several previously reported cancer-specific hypermethylated loci49. For example, the promoter region and multiple distal regulatory elements near the ITGA4 gene are accessible in normal colon, unaffected FAP...
Among the 14 pld mutants with septation defects, 12 mapped to known loci: cdc7, cdc15, cdc11 spg1, and sid2. One of the pld mutants, cdc7-PD1, was selected for suppressor analysis. As multicopy suppressors, we isolated four known genes involved in septation in fission yeast: spg1(+)...
Transcriptome analysis of aphid heads. Samples were dissected heads from AVR (PS01) and VIR (N116) genotypes infested on resistant (R; A17) or susceptible (S; DZA315.16)Medicago truncatulagenotypes for 24 h, withn = 3 biological replicates. Aphid genotype PS01 displays an incompatible in...
More than 800 million people suffer from kidney disease, yet the mechanism of kidney dysfunction is poorly understood. In the present study, we define the genetic association with kidney function in 1.5 million individuals and identify 878 (126 new) loci
1. What type of reasoning is utilized when drawing conclusions from descriptive research? 2. Can descriptive research be used to test hypothesis? Explain why or why not. 3. What are some of the limitations associated with descriptive research? (a) What are polymorphic loci? (b) Give an exam...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of COVID-19, generates multiple protein-coding, subgenomic RNAs (sgRNAs) from a longer genomic RNA, all bearing identical termini with poorly understood roles in regulating
Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene have been recognized as genetic risk factors for Parkinson’s disease (PD). However, compared to cancer, fewer genetic mutations contribute to the cause of PD, propelling the search for protein b
repeats, typically 21–48 bases long, punctuated by short non-identical ‘spacers’ that provide the immune ‘memory’ of these systems. [1–6]. CRISPR-Cas function requires a suite of Cas proteins encoded bycasgenes, which are often located nearby the CRISPR loci (for reviews see [4–11...
microRNAs putatively downregulated due to promoter hypermethylation, high-throughput technologies such as methylation-array analysis are able to simultaneously pinpoint putative candidates [17] and the reliability of the results might be readily assessed through analysis of well-known hypermethylated loci. In...
We generated 46 isogenic HT-29 base-edited cell lines and performed next-generation sequencing of endogenous genomic loci before and after editing (Extended Data Fig. 10a). Amplicon sequencing demonstrated efficient installation of nonsynonymous edits exceeding 10% variant allele frequency (VAF) for 43...