a, UMAP plots of benign breast epithelial cells and BC cells for all snRNA (left) and snATAC (right) samples. Tumor cells colored by PAM50 subtype.b, Heatmap of top 15 DEGs in snRNA-seq data from benign breast duct cells. A subset of genes from each benign cell type is highlighted ...
J. et al. A genetic screen using the PiggyBac transposon in haploid cells identifies Parp1 as a mediator of olaparib toxicity. PLoS ONE 8, e61520 (2013). Article CAS PubMed PubMed Central Google Scholar Gill, S. J. et al. Combinations of PARP inhibitors with temozolomide drive PARP1 ...
Inflammation is known to exacerbate AD pathogenesis [4]. Therefore, several research groups have proposed to treat AD with anti-inflammatory drugs. In fact, nonsteroidal anti-inflammatory drugs (NSAIDs) have been shown to delay dementia in AD [5]. NSAIDs are some of the most widely used dru...
Systemic sclerosis: one year in review 2023 2023, Clinical and Experimental Rheumatology A single-cell multiomic analysis of kidney organoid differentiation 2023, Proceedings of the National Academy of Sciences of the United States of America Immune cell dysregulation as a mediator of fibrosis in system...
Mediator and cohesin connect gene expression and chromatin architecture Nature, 467 (2010), pp. 430-435 CrossrefView in ScopusGoogle Scholar Kent et al., 2002 W.J. Kent, C.W. Sugnet, T.S. Furey, K.M. Roskin, T.H. Pringle, A.M. Zahler, D. Haussler The human genome browser at...
Since KLF4 was not only an in vivo-specific hit from our screen, but also a likely mediator of the in vivo-specific chromatin changes observed, we chose to start with this factor. Using the top sgRNA from our screening library, we knocked out KLF4 in a pooled format in MG63.3-GFP-...
Some enhancers were insensitive to the depletion of the core Mediator subunit MED14 or the bromodomain protein BRD4 and regulated distinct transcriptional programmes. In particular, canonical Mediator7 seemed dispensable for P53-responsive enhancers, and MED14-depleted cells induced endogenous P53 target ...
Our work challenges the generally assumed prevalent role of miRNA as sole mediator of exRNA intercellular communication and points to the need for further in-depth investigation into other classes of exRNAs. Our studies also demonstrate the importance of better defining heterogeneity at the molecular ...
in PWK/PhJ is the same as that in C57BL/6J, howeverNlrp1cis a predicted pseudogene. We found an 18 amino acid mismatch in the nucleotide-binding domain (NBD) betweenNlrp1b_CAST andNlrp1b_PWK. These divergent profiles suggest thatNlrp1cis not the sole mediator of anthrax lethal toxin ...
identified the mechanoresponsive ion channel Piezo1 as a key mediator of oligodendrocyte progenitor cell ageing, which corresponds to the fact that the prefrontal cortex progressively stiffens during senescence11. For bone marrow-derived mesenchymal stem cells, their ageing is regulated by autophagy ...