Checkpoint immunotherapy (CPI) has increased survival for some patients with advanced-stage bladder cancer (BCa). However, most patients do not respond. Here, we characterized the tumor and immune microenvironment in pre- and post-treatment tumors from the PURE01 neoadjuvant pembrolizumab immunotherapy ...
Magnification: 100×. (B) Effect of MIP-1γ on NF-κB activation in osteoclasts. Osteoclasts were generated by RANKL stimulation of RAW264.7 cells for 5 days, washed, and re-stimulated with RANKL (10 ng/ml) (lane 4), MIP-1γ (10 ng/ml) (lane 5) or medium (lane 3) as a ...
For each tumor section, quantification of immunofluorescence double staining was performed by counting Ki-67+ cells in six high power fields (400 × magnification) in parallel with LgR5+. The proportion of Ki-67 positivity in counted LgR5+ cells was expressed in percentages. Real-time quantitativ...