A 10 mV junction potential compensation was applied to correct the liquid junction potential between pipette solutions relative to extracellular solution. All whole-cell currents were filtered at 3.3 kHz and sampled at 10 kHz. Western analyses. Cells were lysed in cell lysis buffer containing 50 mM...
Answer the following question. Why do we say a substance in a liquid phase is more disordered than the same substance in the solid phase? Which phase of matter consists of particles that are locked into place and unable to move past each other? What do we understand by a balanced and unb...
Porous vs Non-porousA porous material is one that can hold liquid or air, while a non-porous surface does not have any internal openings and therefore cannot hold liquids or air. Some examples of porous materials are a sponge or cork. ...
Cells in 1 mL liquid culture were pelleted and lysed with 20 μL SDS loading buffer. Cells were incubated at 98°C for 10 min and then on ice for 2 min. Whole lysate was separated on a 15% SDS-PAGE gel and blotted onto a nitrocellulose membrane (Bio-Rad). The blots were incubated...
Thymic tissue from CXCL12dsRedmice was treated for 2 hours with 2% PFA followed by overnight treatment with 18% sucrose prior to snap freezing. Freshly isolated thymic tissue from non-fluorescent reporter mice was mounted in OCT and snap frozen in liquid nitrogen. 8μm cryosections were prep...
(Fig.3h, top) were expressed in 293 T cells, and subjected to in-gel tryptic or chymotryptic digestion and liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) by parallel reaction monitoring (PRM) that interrogates the fragmentation of specific ions. One tryptic peptide, NYIAQ...
The final eluate was subjected to liquid chromatography-mass spectrometry (LC-MS), and the spectrum count of each protein is color-coded and displayed as a heat map, with more intense color representing more abundant species. Polypeptides are grouped according to which PCGF they associate with (...
(Illumina) using both, Sciclone and Zephyr liquid handling robotics (PerkinElmer). Library concentrations were quantified with the Qubit 2.0 Fluorometric Quantitation system (Life Technologies) and the size distribution was assessed using the Experion Automated Electrophoresis System (Bio-Rad). For ...
Liquid chromatography and tandem mass spectrometry Mass spectrometry data were collected using an Orbitrap Fusion or Lumos mass spectrometer (ThermoFisher Scientific, San Jose, CA) coupled to a Proxeon EASY-nLC 1200 liquid chromatography (LC) pump (ThermoFisher Scientific, San Jose, CA). Peptides ...
The vehicle and crizotinib-treated tumours (collected 2-h post treatment) were grinded in liquid nitrogen and lysed in ice-cold RIPA buffer (Thermo Scientific, Waltham, MA, USA). The protein concentration was determined by BCA (Pierce, Rockford, IL, USA). In all, 80 μg of tumour ...