The models have been implemented using Python version 3.9.16 and with the support of PyTorch 1.13.1, CUDA 11.9, and Scikit-learn 1.2.1. All the experiments have been executed on a workstation with an Intel i9 CP
The label-free quantitation node of Precursor Ions Quantifier in the consensus workflow through the Proteome Discoverer v2.3 (Thermo Fisher Scientific) was used to quantify all data. For the quantification of proteomic and phosphoproteomic data, the intensities of peptides were extracted with initial p...
Parallel Reaction Monitoring-Mass Spectrometry (PRM-MS) and Western blot are both commonly used for the initial validation of potential biomarkers. We selected several of our top disease biomarkers and urinary exosome markers for further validation. Quantitative assays based on PRM-MS for the disease...