hybridization has been increasingly studied for its evolutionary and conservational implications on the long-term survival of the involvedtaxa1,2,3,4,5. However, while natural hybridization between closely relatedtaxais frequently acknowledged as an evolutionary process providing novel adaptive gene assembla...
For PURE01, DNA was extracted from FFPE tissue from pretherapy transurethral resection of the bladder (TURB) samples, and a hybridization-capture panel of approximately 400 cancer-related genes was sequenced to a median coverage of 743x, then analyzed for mutations and copy number alterations by ...
We have used a combination of gene expression microarrays and array comparative genomic hybridization (aCGH) to show that meningiomas of all three grades fall into two main molecular groups designated 'low-proliferative' and 'high-proliferative' meningiomas. While all benign meningiomas fall into the ...
While a more broad and inclusive definition may initially cause some confusion, a hybridization of the definitions widely accepted throughout the world should help consolidate and focus the unique regional aspects of SSS. We therefore recommend adopting the following definition of short sea shipping: ...
Sample library preparation, hybridization, and quality control were performed according to the manufacturer’s protocols. Expression data were normalized using the MAS5.0 algorithm of the GCOS program (Affymetrix Inc). Gene expression data of additional adult glioblastoma samples (n = 74) were obtained...
Fluorescence In Situ Hybridization (FISH) and Immunofluorescence For FISH/IF, transfected DRG neurons were fixed for 15 min in 4% paraformaldehyde (PFA). Digoxigenin (DIG) labeled GFP antisense cRna probes were used to detect GFP as described; DIG labeled sense probes were used for control (26...
Of the 478 children with ASD, 464 (97.1%) children underwent genetic testing (karyotyping and/or array comparative genomic hybridization (aCGH), and/or genome-wide association (GWA)). A total of 42 (8.8%) children had a known possible genetic or non-genetic risk factor for autism (39 chil...
In the 2010s, more than ever, we’ve seen pop’s hybridization with rap. The influence is clear in pop singers’ rap-inflected phrasing, song lyrics that borrow words from the hip-hop lexicon, bass-heavy beats and, of course, guest verses from the MC du jour. For the past four ...
Samples were sent to the CAP/CLIA (College of American Pathologists and Clinical Laboratory Improvements Amendments) accredited central laboratory at Nanjing Geneseeq Technology Inc. (Nanjing, China) for genomic DNA extraction and hybridization capture-based targeted NGS of 422 cancer-relevant genes. Prot...
Data from RT-PCR, in situ hybridization, and immunohistochemical analysis have demonstrated that the 26RFa-encoding gene is primarily expressed in the brain, particularly in the hypothalamus in humans and rodents [[1], [2], [3], [4], [5],7]. However, 26RFa mRNA is also detected in ...