These adapters contained the full complement of sequencing primer hybridization sites for single, paired-end, and indexed reads. Single- or dual-index adapters were ligated to the fragments, and the ligated products were amplified by the polymerase chain reaction. After library purification, ...
Nakaseomycesis a clade of Saccharomycotina yeasts, sister to theSaccharomyces genus, and currently formed by nine described species, of which six are environmental (Nakaseomyces delphensis,Nakaseomyces castellii,Nakaseomyces bacillisporus,Nakaseomyces uthaithaninus,Nakaseomyces kungkrabaensisan...
Samples were sent to the CAP/CLIA (College of American Pathologists and Clinical Laboratory Improvements Amendments) accredited central laboratory at Nanjing Geneseeq Technology Inc. (Nanjing, China) for genomic DNA extraction and hybridization capture-based targeted NGS of 422 cancer-relevant genes. Prot...
namely, Orf10 polypeptide is not required for stimulation of translation. In view of the proximity ofORF10to the 3′-terminus, its seminal contribution to HVR structure, its unique presence in sarbecoviruses, and the uncertainty of the function (and even expression) of Orf10 polypeptide37,38,...
Gene Expression Omnibus: NCBI gene expression and hybridization array data repository Nucleic Acids Res., 30 (2002), pp. 207-210 View in ScopusGoogle Scholar [22] P.C. Grayson, S. Eddy, J.N. Taroni, Y.L. Lightfoot, L. Mariani, H. Parikh, et al. Metabolic pathways and immunometabolism...
"Ken thinks he and Becky are just having fun together. Becky is already picking out bridesmaid dresses. Melissa has decided to break up with Tony. Tony thinks Melissa is "the one." "Are miscommunications like this unavoidable? What's the best way to end a relationship-or to take it to...
Alveolar epithelial type 2 cells (AEC2s) are the facultative progenitors responsible for maintaining lung alveoli throughout life but are difficult to isolate from patients. Here, we engineer AEC2s from human pluripotent stem cells (PSCs) in vitro and use time-series single-cell RNA sequencing ...
Hybridization of samples targeting predetermined regions of the genome provided by Twist Target Enrich- ment Protocol (Twist Bioscience) using Twist Hybridi- zation and Wash kit (Twist Bioscience). The probes are labeled with biotin and streptavidin-conjugated mag- netic beads (Lablife Nordic) ...
Finally, we analysed a different transgenic mouse line, expressing CreER under control of a 2.7 kb region of the Fezf2 promoter29, to corroborate Fezf2-driven GFP expression in the BAC transgenic line, as well as Fezf2 in situ hybridization data available from the Allen Brain Atlas27. Fezf2...
We have used a combination of gene expression microarrays and array comparative genomic hybridization (aCGH) to show that meningiomas of all three grades fall into two main molecular groups designated 'low-proliferative' and 'high-proliferative' meningiomas. While all benign meningiomas fall into the ...