We quantified enhancer activities along the entire human genome using STARR-seq6 in HCT116 cells, following the rapid degradation of eight cofactors. This analysis identified different types of enhancers with distinct cofactor requirements, sequences and chromatin properties. Some enhancers were ...
User-centred designInternet of ThingsSearch engines play an important role in determining the success of e-commerce. Despite many efforts have been made to improve searching methods (SM) they remain mostly limited to semantic elaboration of keywords. This implies that the SM are not capable of ...
Single-cell suspensions obtained from tissue digests were blocked in PBS 5% BSA containing human anti-Fc block (Miltenyi), except when CD16 was in the panel, stained for FACS analysis or sorting with Fixable Viability Dye eFluor 780 (eBioscience, 1:1,000), DAPI (Invitrogen, 1:50,000) ...
Residue 116 faces the allosteric pocket and is conserved in the human P2XRs. The Q116A mutant had no effect on AZ10606120 sensitivity (Fig. 5). Adjacent to Q116 in the structure is tryptophan 167 that is part of the WCP motif that is conserved throughout all P2XRs. Neither alanine, cys...
The fluorescence profile of the proteins was obtained by integrating the pixel intensity within a narrow window centred on the cell online (Supplementary Fig. 1i). The intensity of all the frames was averaged and used for subsequent analysis (see Supplementary Fig. 3b for details). The averaged...
Central to the roles and responsibilities of staff at Housing Services is the way they work with their clients and honour them as full human beings. The Housing Services program aims to walk alongside their clients on their journey from the street to safe and appropriate housing of their choice...