Alanine scanning;Binding hotspots;Chimeric scanning;Homologue scanning;Interaction interfaces;Mutagenic scanning to define binding hotspots;Mutation Definition Site-directed mutagenesis, originally developed by Hutchinson and colleagues in the 1970s, is an invaluable tool for identifying residues that are importa...
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ML is the branch of computer science that aims at inferring the input-output relationship underlying a collection of examples15. In order to devise such relationship, ML models need to be trained on a set of input-output pairs, where the input data x are described as d-dimensional vectors ...
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Lastly, the study details the signaling pathways present in non-responding patients and finds that they can be divided into two major groups: “TIL-hot” tumors with high expression of negative T cell regulators, e.g., IDO1, ICOS, and TIGIT, who may benefit from further treatment ...
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Nanopore sequencing was performed at the Ultra-sequencing core facility of the Centro Nacional d’Analisi Genòmica (CNAG) in Barcelona (Spain). Integrity of genomic DNA was analyzed by pulse field electrophoresis (Pippin Pulse, Sage Science), and contamination of DNA samples was checked with NanoD...
9B and demonstrate that this mutation is strikingly cyh-sensitive, as the spot tests suggested. The difference in IC50 values between the N242K mutant and the isogenic WT control is ∼3-fold. The minimum inhibitory concentrations of cyh for the N242K mutant and WT strains are 4.2 and ...
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