Male reproductive system ageing is closely associated with deficiency in testosterone production due to loss of functional Leydig cells, which are differentiated from stem Leydig cells (SLCs). However, the relationship between SLC differentiation and age
Assertion : The concentration of sulphide ore by froth floatation process is based on emulsification. Reason : The addition of pine oil in w ater froms an emulsion. View Solution Doubtnut is No.1 Study App and Learning App with Instant Video Solutions for NCERT Class 6, Class 7, Class 8...
(E3889; Sigma-Aldrich), 0.5% Triton-X-100 (Sigma-Aldrich) and 1 mM dithiothreitol (DTT; D0632; Sigma-Aldrich) supplemented with protease inhibitor cocktail (P9340; Sigma-Aldrich) or lysed in 2× sample buffer (125 mM Tris-HCl, pH 6.8, 4% SDS, 20% glycerol, 200 mM DTT and...
Studies with a water-soluble diacylglycerol, sn-1,2-dibutyrylglycerol, allowed direct comparison of diacylglycerol kinase activity in mixed micelles with that in native membranes. The results are discussed in relation to the structural requirements of other diacylglycerol-dependent enzymes....
Then the cells were mounted in 50% glycerol and visualized by fluorescence microscopy using a Zeiss Axiovert 200 microscope and linked to a LSM5 computer system. Excitation was performed at 488 nm, and fluorescence detection used a 505–530 nm bandpass filter. The plasma membrane localization of...
(bottom).eSupershift RNA EMSA assay ofdfraction 16 from spike S1- and IFN-γ-treated cells with antibodies against MSC constituents; two different anti-RARS1 and anti-KARS1 antibodies were used (top). Free probe at lower exposure (bottom). Vehicle is 50% glycerol in PBS. Experiment inb...
We sequenced seven strains ofN. bracarensisidentified as CBM1 to CBM7 using short read technology (Table3) [5,22,43]. Strains were stored in 50% glycerol at -80 C. Culturing of the strains involved plating a smear of biomass on YPD agar plates and incubating for 2 days at 30 ºC...
The activity is mediated by the N-terminal domain whose crystal structure revealed a large hydrophobic cavity capable of binding ~10 glycerolipid molecules59. In yeast, Vps13p has been identified at membrane contact sites connecting endoplasmic reticulum, mitochondria, endosomes, and vacuoles60,61 ...
U2OS cells carrying latent HHV-6A treated with solvent control or TSA were lysed in RIPA buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% Triton X-100, 1% NP-40, 0.1% SDS, 10% Glycerol and protease inhibitor cocktail (Roche)). Protein lysates were incubated with control...
The widespread presence of RYBP and YAF2 indicates that they play a critical role in the function of PRC1 complexes. In fact, inclusion or exclusion of RYBP/YAF2 results in additional heterogeneity within the six PRC1 complex groups, as evidenced by glycerol gradient fractionation followed by ...