Autoantibodies against cardiac G-protein-coupled receptors define different popula- tions with cardiomyopathies but not with hypertension. Clin Immunol Immunopathol 1994; 72: 15-20.Fu ML, Hoebeke J, Matsui S, Matoba M, Magnusson Y, Hedner T, et al. Autoantibodies against cardiac G-protein-...
Biased signaling G-protein coupled receptor Gβγ subunits Naturally occurred mutants Protein kinase C 1. Introduction The neuropeptide 26RFa, also known as pyroglutamine RF-amide peptide (QRFP), contains 26-amino acid residues. It is the most recently identified member of the RF-amide peptide fa...
Studies have reported clinical heterogeneity between right-sided colon cancer (RCC) and left-sided colon cancer (LCC). However, none of these studies used multi-omics analysis combining genetic regulation, microbiota, and metabolites to explain the site-
We transduced cells with the gRNA virus (plus 8 µg ml−1 polybrene; ThermoFisher Scientific) to achieve an infection rate of ~30%, as measured by blue fluorescent protein (BFP) fluorescence with flow cytometry. We performed all base editing screens at ~1,000× coverage (estimated ...
More than 800 million people suffer from kidney disease, yet the mechanism of kidney dysfunction is poorly understood. In the present study, we define the genetic association with kidney function in 1.5 million individuals and identify 878 (126 new) loci
000 × gfor 5 min to pellet cellular debris. The supernatant was removed and quantified, and 100 µg of protein was purified using a 2D Clean-up Kit (GE HealthCare) following the manufacturer’s instructions with the exception that 400 μl of precipitant and co-precipitant were ...
epidermal growth factor receptor; FGF, fibroblast growth factor; HGF, hepatocyte growth factor; HMGB1, high mobility group protein 1; IGF, insulin-like growth factor; IL, interleukin; NOTCH3, neurogenic locus notch homolog protein 3; LOXL2, lysyl oxidase like 2; MMP, matrix metalloproteinase; PD...
Tyrosine kinase-mediated signal transduction pathways and the actions of polypeptide growth factors and G-protein-coupled agonists in smooth muscle This synopsis focuses on the role that tyrosine kinase pathways may play in the acute regulation of smooth muscle contractility by receptor-kinase-activati....
This is known as hysteresis and is driven by limited access to the site of drug action or slow receptor kinetics. In the main, current drug discovery programs are only able to truly investigate concentration-effect-time relationships during the advanced stages of the preclinical program. At this...
Specific interaction with rhodopsin is dependent on the γ subtype in a G protein J. Biol. Chem. (1993) P. Butkerait et al. Expression of the human 5-hydroxytryptamine 1A receptor in Sf9 cells. Reconstitution of a coupled phenotype by co-expression of mammalian G protein subunits J. Biol...