In general, Spanish forests are predominantly privately owned (approx. 70%) and exhibit high spatial fragmentation, which significantly hinders effective long-term forest management planning [38, 39]. Conceptual design The main objective of the study is to define and spatially assign a FUS to each...
3h, top) were expressed in 293 T cells, and subjected to in-gel tryptic or chymotryptic digestion and liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) by parallel reaction monitoring (PRM) that interrogates the fragmentation of specific ions. One tryptic peptide, NYIAQVDVVNFN...
76.9 months in the gefitinib group vs 67.1 months in the VP group; HR 0.87 (95% CI 0.57–1.35),P = 0.54) (Fig.3aand Supplementary Fig.5). Promisingly, MINERVA successfully demonstrated the stratification of OS benefit as well. In ...
Anthropogenic pressures in the recent past have led to severe population declines1 and substantial fragmentation of wildlife populations2. As a result, small and fragmented wildlife populations are more vulnerable to genetic drift and inbreeding3, which, in turn may reduce their fitness and evolutionary...
Each fraction was injected into the Orbitrap Fusion Lumos (Thermo Scientific) operating in data-dependent mode with quadrupole isolation and HCD fragmentation. MS1 resolution was set to 60k and MS2 resolution was set to 30k. Each fraction was injected twice, with the first injection scanning from...
RNA fragmentation reagents Ambion Cat # AM8740 T4 RNA Ligase 1 (ssRNA Ligase) NEB Cat # M0204 AffinityScript Multi-Temp RT Agilent Cat # 600109 Kapa HiFi HotStart PCR ReadyMix Kapa Biosystems Cat # KK2601 Whole Skin Dissociation Kit, human Miltenyi biotec Cat # 130-101-540 Gentlemacs dissoci...
(Tohge et al., 2016); and finally, (5) the full analytical capacity of the machine was exploited by acquiring four different mass spectra, i.e., in positive/negative ion detection mode and with/without in-source fragmentation, which were used for peak annotation and identification (...
Anthropogenic pressures in the recent past have led to severe population declines1 and substantial fragmentation of wildlife p opulations2. As a result, small and fragmented wildlife populations are more vulnerable to genetic drift and inbreeding3, which, in turn may reduce their ...
Protected areas and forest fragmentation: Sustainability index for prioritizing fragments for landscape restoration. Geol. Ecol. Landsc. 2021, 5, 19–31. [Google Scholar] [CrossRef] Terêncio, D.P.S.; Varandas, S.G.P.; Fonseca, A.R.; Cortes, R.M.V.; Fernandes, L.F.; Pacheco, F....
LC-MS/MS analysis identified a novel indole-diterpene at 32.8 min within the wild-type sample with a peak at m/z 504.3 that is absent in ΔpaxD (Figure 9a,b). Based on MS analysis this compound is proposed to be an isoprenylated derivative of paxilline. MS2 fragmentation of this ion ...