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We designed the pegRNAs using PRIDICT as above and filtered for pegRNA inserts that were <200 bp in length after the addition of Gibson homology arms for cost-effective DNA oligo synthesis (Twist Biosciences). A library of 162 vectors was cloned using Gibson assembly (NEB), to generate a...
We employed both ten-fold cross validation as well as LOOCV methods (as internal validation procedures) to evaluate the robustness of our MINERVA score. For each fold of cross validation, a subset of markers was selected based on interactionP < 0.05, which were then used to build mock M...
) A free fold of the peritoneum, or one serving to connect viscera, support blood vessels, etc.; an epiploon. Omer (n.) A Hebrew measure, the tenth of an ephah. See Ephah. Omiletical (a.) Homiletical. Ominate (v. t. & i.) To presage; to foreshow; to foretoken. Omination...