e Representative transmission electron microscope (TEM) images of primary Nestin-GFP + SLCs from different age groups. Scale bar, 250 nm for original pictures and 100 nm for enlarged pictures. f Quantification of the extent of MERCs (<100 nm) from (e) in primary Nestin-GFP +...
This study aims to understand tumor heterogeneity and its relation to BC lineage at a single-cell resolution. Chromatin accessibility, TF motif enrichment and their impact on the transcriptome reveal the structure of BC heterogeneity through integration of bulk-RNA/DNA sequencing, scRNA-seq, snRNA-se...
) The eyepiece of an optical instrument, as of a telescope or microscope. Ocularly (adv.) By the eye, or by actual sight. Oculary (a.) Of or pertaining to the eye; ocular; optic; as, oculary medicines. Oculate (a.) Alt. of Oculated Oculated (a.) Furnished with eyes. Ocula...
Staining for LgR5, Cdx-2, and Ki-67 was performed on serial sections of 2 μm thickness. Tissue sections were cut from formalin-fixed paraffin-embedded (FFPE) blocks on a microtome and mounted on adhesive microscope slides (Hartenstein, Wuerzburg, Germany). For immunohistochemistry, unconjugated...
For visualization by TEM, the eluted vesicles were adsorbed to Formvar-carbon coated electron microscopy grids, washed with PBS and imaged on Tecnai G2 Spirit BioTWIN microscope at 80 kV. Ultracentrifugation Like for the filter fractionation, conditioned media was cleared of cells as above and EVs...
Oncogene-induced senescence was assessed by staining with the β-galactosidase staining kit 48 h after drug treatment in 96-well plates following manufacturer’s instructions (Cell Signaling Technology, cat. no. 9860) and images were acquired on the EVOS XL Core microscope (ThermoFisher Scientific...
Oncogene-induced senescence was assessed by staining with the β-galactosidase staining kit 48 h after drug treatment in 96-well plates following manufacturer’s instructions (Cell Signaling Technology, cat. no. 9860) and images were acquired on the EVOS XL Core microscope (ThermoFisher Scientific...
fixation timepoints, which showed we could maximize differences between cell lines under these parameters (Fig.S1D). Each screening plate was stained with the standard Cell Painting dyes and imaged on a Perkin Elmer Phenix automated microscope within 48 h. We implemented this same workflow across ...
Collagen was visualized by using either DIC internal reflection within the confocal microscope or GFP-CNA35 probe (1:1000) [17]. The following secondary antibodies were used for immunofluorescence: Alexa-fluor-488/568/647-conjugated goat anti-mouse or goat anti-rabbit antibodies (1:500, ...
Unstained formalin-fixed and paraffin-embedded (FFPE) bladder tissue/tumor sections were received from the PURE-01 study. A representative slide from each patient was stained with hematoxylin and eosin. Images were taken with a Zeiss Axioskop/Nuance microscope using a 10x objective. ...