Application of TraDIS to define the core essential genome of...
2 μg ofCampylobactergenomic DNA was incubated with 1 μg of pRC1-marinerand thehimartransposase in transposition buffer (40% glycerol, 80 μM DTT, 100 mM HEPES, pH 7.9, 1 mg/ml BSA, 400 mM NaCl2, 20 mM MgCl2).