Therefore, we next sought to identify subpopulations of T cells associated with response, by performing mass cytometry (CyTOF) on baseline (PRE) melanoma tissue dissociates from patients treated with anti-PD-1 monotherapy (n = 11) or combined immunotherapy (n = 7), using a customized panel ...
to dissociate organoids into single cells. Single cells were resuspended in FACS buffer (0.5 mM EDTA and 5% FCS) and individual viable cell (DAPI negative) were seeded into three 384 well plates per condition. Single-cell mRNA-sequencing was performed by Single Cell Discoveries (Utrecht, ...
Importantly, ESCRT-0 dissociates simultaneously, and the concerted release of ESCRT-0, -I and ESCRT-III is required for efficient ILV formation, as demonstrated by the impaired ILV formation in the hyperstable HRS770 mutant. Therefore, this second phase most likely corresponds to the formation ...