Just as average expression of PAM50 genes in tumor cells from snRNA-seq could discriminate tumor subtype, chromatin accessibility in the promoters of PAM50 genes from snATAC-seq showed good segregation of tumors by subtype in the 21 samples (from 21 cases) with both data types available (Fig....
In total, we determined a panel of unique proteins and phosphoproteins as novel high-confidence candidates for disease biomarkers. Disease biomarkers will help diagnose whether a patient currently has PD. Our large-scale LC-MS analysis efforts combined with extensive bioinformatics analysis led to the...
In total, we determined a panel of unique proteins and phosphoproteins as novel high-confidence candidates for disease biomarkers. Disease biomarkers will help diagnose whether a patient currently has PD. Our large-scale LC-MS analysis efforts combined with extensive bioinformatics analysis led to the...
N protein is among the most abundant SARS-CoV-2 proteins9. As ORFNis immediately upstream ofORF10inNsgRNA, translation ofNcould disrupt RNA structures driving internal initiation ofORF10, thereby facilitating re-initiation ofORF10in the 3′-UTR, as anNsgRNA-specific route forORF10translation41. ...
To prepare aggregates of model substrates, firefly luciferase (FFL; 10 µM) was denatured in 7 M urea in refolding buffer (25 mM HEPES-KOH pH 7.5, 150 mM potassium acetate, 10 mM magnesium acetate, and 10 mM DTT) for 30 min at 22 °C. β-galactosidase (β-gal...