only recently a specific field dealing with organelle communication at membrane contact sites has gained wide acceptance, attracting scientists from multiple areas of cell biology. The diversity of approaches warrants a unified vocabulary for the field. Such definitions would facilitate laying the foundatio...
Synovial mesenchymal stem cells (MSCs) have high freeze–thaw tolerance, whereas human umbilical vein endothelial cells (HUVECs) have low freezing tolerance. The differences in cell type-specific freeze–thaw tolerance and the mechanisms involved are unc
Vesicle-mediated trafficking of parasite proteins to the host cell cytosol and erythrocyte surface membrane in Plasmodium falciparum infected erythrocytes. During the development of the asexual stage of the malaria parasite, Plasmodium falciparum, the composition, structure and function of the host cell me...
For physical analysis, the cell membrane fluidity of MSCs and HUVECs before and after DMSO addition was assessed using a histogram for generalized polarization frequency. Results:HUVECs showed lower live cell rates and higher gene expression alteration related to extracellular vesicles in response to ...
CA9 protein expression was observed in the membrane of tumor cells in two of nine non-responding cases via immunohistochemistry (Figure S2B). Notably, perturbed genes in the known immunosuppressive WNT- and mammalian target of rapamycin-signaling pathway (WNT3; adjusted p = 0.015), and cell ...
Gαh/transglutaminase-2 activity is required for maximal activation of adenylylcyclase 8 in human and rat glioma cell Gαh (or transglutaminase-2 (TG2)) is an atypical guanine nucleotide binding-protein that associates with G protein-coupled receptors. TG2 also exerts tran... Yutaro,Obara,Yosh...
To quantify cellular traits, we adopted the Cell Painting assay17,18. This multiplexing dye assay uses six stains to capture morphological characteristics for eight cellular compartments: Hoechst 33342 (DNA), wheat germ agglutinin (WGA) (golgi and plasma membrane), concanavalin A (endoplasmic reticulu...
Cell 141, 656–667 (2010). Article CAS PubMed PubMed Central Google Scholar Korobova, F., Ramabhadran, V. & Higgs, H. N. An actin-dependent step in mitochondrial fission mediated by the ER-associated formin INF2. Science 339, 464–467 (2013). Article ADS CAS PubMed Google Scholar...
The vesicle-sequestered arsenate is reduced by PvGSTF1 and perhaps PvACR2 and delivered to the vacuole for storage through membrane fusion. Arsenite enters the cell by PvTIP4, where it is pumped into the vacuole for storage by PvACR3 [12]. The coupling of the GAPDH product 1-As-3-PG ...
(approximately 150 ml). Pooled diets were concentrated using a Vivacell 250 Pressure Concentrator (Sartorius Mechatronics, UK) using a 5000 Da molecular weight cut-off (MWCO) polyethersulfone (PES) membrane. When the final volume had reached 5 ml it was removed and 1 ml of filtered sterilised...