For nrf-6::gfp, the GFP coding region was generated by PCR from pPD95.69 (A. Fire) and inserted at the BstBI site (Arg-819) in the nrf-6 cDNA. A 2.8 kb NotI–BlpI genomic fragment containing 2.3 kb of nrf-6 promoter, exon 1, intron 1, and part of exon 2 was then fused to...
Insemination in scorpions is carried out by means of a partly sclerotized structure, the spermatophore, which is composed of two separate halves, the hemispermatophores. In most genera these reproductive structures can be used to differentiate species. However, many taxa such as the genusEuscorpius...
A third example consistent with this model is the AvrRxv/AvrBsT family of AVR proteins, which belong to the YopJ family of cysteine proteases Barrett and Rawlings 2001, Orth et al. 2000. As with the AvrPphB and AVR-Pita, mutation of the putative catalytic residues of AvrBsT eliminates ...