Emerging single cell sequencing analyses however point toward a crucial role for endothelial cells derived from the venous domain in shaping the angio-architecture, in particular in the brain25,27. Yet these systems biology findings await genetic and functional confirmation. Alterations in venous ...
Along each of these paths, the differentiating cell encounters branch points that end in different tissues, with the apex of this branch point representing intermediate cell states. In a similar way, we envision a metastasizing cell as rolling down an analogous epigenetic landscape. Where fully ...
To overcome this, we first identified a set of genes for each cell type that showed evidence of differential expression (p-value < 0.1) between a post-infection and pre-infection time point. Next, we defined the global perturbation vector as the average log-fold change of each DEG relative...
A central aim of developmental biology is to better understand the embryonic differentiation and maturation pathways that lead to functioning adult cells and tissues. Multistage, stepwise differentiation protocols applied to cultured human PSCs are designed to recapitulate these pathways in order to produce...
However, only recently a specific field dealing with organelle communication at membrane contact sites has gained wide acceptance, attracting scientists from multiple areas of cell biology. The diversity of approaches warrants a unified vocabulary for the field. Such definitions would facilitate laying ...
Total axon length (A) and branching (B; branch point number/per neuron) are shown as mean fold change versus siCon ± S.E. (N ≥ 200 neurons in three independent cultures; *, p≤ 0.05, **, p≤ 0.01, ***, p < 0.005 by one-way ANOVA with pair-wise comparison and Tukey post-...
articles Pre-mRNAsplicingtakesplaceinthespliceosome,consistingof U1,U2andU4/U6•U5smallnuclearribonucleoproteinparticles (snRNPs) 1 .U1definesfunctional5′splicesites(5′SSs)whereasU2 recognizesfunctional3′splicesites(3′SSs)bybase-pairingwith thebranch-pointsequence(BPS).BecausetheBPSisquitedegen- ...
Each point corresponds to an individual pancreatic cancer sample with a hotspot KRAS mutation. Extended Data Fig. 5 Inferred mutant immunogenicity is not related to pathogenicity in non-cancer driver genes. a–f, Comparison of inferred immunogenicity across not-pathogenic and pathogenic missense ...
RNA splicing shapes the gene regulatory programs that underlie various physiological and disease processes. Here, we present the SCASL (single-cell clustering based on alternative splicing landscapes) method for interrogating the heterogeneity of RNA spl
2e) which also failed to generate effective resolution of biology between modules. Thus, we concluded that a combination of edge reduction with the FastUnfold approach enhanced the resulting network solution in gene correlation data. Next to assess whether EPG edge reduction with FastUnfold provides ...