- 《Blood Advances》 被引量: 0发表: 2022年 NPM1-Mutated Myeloid Neoplasms with Nucleophosmin (NPM1) gene mutations rarely occur in non-acute myeloid neoplasms (MNs) with <20% blasts. Among nearly 10,000 patients investigated so far, molecular analyses documented NPM1 mutations in around 2% ...
Immunoprecipitation for molecular weight determination was also performed centrally with T-cell blasts and a B-cell line as the sources of antigen. Clusters of three or more antibodies were found that defined the canine equivalents of the CD5, CD4, CD8 and Thy-1 antigens, and these could be...
This suggests that C2 cells may indeed be an intermediate stage during the malignant transformation of normal epithelial cells into cancerous cells in TNBC. Interestingly, such similarity between C2 and normal epithelial cells cannot be seen from the gene expression profiles (Fig.2F), suggesting that...
if they had B-phenotype ALL in second or greater relapse or in first relapse with one or more of the following high-risk features: age <1 or >18 years at diagnosis; t(9;22); first remission <18 months; ≥60% blasts in the bone marrow or ≥30% blasts in the peripheral blood. Onl...
mutDNMT3a and mutNPM1 were found together only in granulocyte monocyte progenitor (GMP) and CD33+ blasts. Importantly, even for patients in whom mutDNMT3a was not detected in mature lymphoid populations, mutDNMT3a without mutNPM1 was found in MPP, MLP, CMP, providing strong evidence that ...
We used nuclease accessibility sequencing in cell lines as well as patient blasts to identify accessible DNA elements and identi-fied > 100 000 accessible regions in each case. Using ChlP-seq, we Identified H2A.Z as a histone modification generally asso-ciated with these accessible regions, ...
Next-generation sequencing was performed at diagnosis in 22 patients. Median age was 62 years (range, 19-86). Twenty-four patients (77%) had normal karyotype, and all had multilineage dysplasia. Most patients could be classified as MDS with excess blasts (19/31, 61%). NPM1 mutations were...
Importantly, western blot analysis of primary leukemic blasts revealed expression of only the 28kDa C-terminal ERG protein, along with full length ERG expressed from the retained wild type allele. Remarkably, the C-terminal ERG protein was also detected in 4 of 5 novel ALL cases that lacked ...
Multivariate analysis of pre-imatinib factors identified detectable peripheral blood (PB) blasts and an interval of more than 2 years from diagnosis as being adverse factors for the detection of mutations. This analysis was used to generate low, intermediate and high risk groups with estimated ...
"The technology we currently use can't identify specifically what's wrong," Kolhe said. Patients have high percentages of cancer-producing cells called blasts in their blood and bone marrow but they do not show the distinctive chromosomal alterations that typically help characterize the leukemia and...