whereNis the number of spectra measured for each sample or blank,\({x}_{i,j}\)is the signal of HR fragment ions in each spectrum and\(\bar{{x}_{i}}\)is the average signal of HR fragment ions(j)for each sample(i)or blank (i). Ions with signal-to-noise ratio (SNR) less th...
or atomic force microscopy95can be powerful tools to accurately visualize them and study the localization and the distribution of the proteins that reside within these structures. Super-resolution methods that can be used include structured illumination microscopy, stimulated emission depletion (STED) micr...
while others may depend on Hsp70/40 to be recruited. Thus, in addition to its role as an Hsp104 activator, Hsp70 may serve as an adaptor to increase the Hsp104 substrate spectrum. Our findings also resolve the outstanding question regarding the functional role ...
Raman Imaging: Raman imaging was performed on an NT-MDT Spectrum Instruments Ntegra II Raman–AFM microscope system (NT-MDT, Russia) equipped with a 60×/1.0 NA water immersion objective (Olympus, Japan). The light source was a 532 nm laser with a power of 12 mW above the objective. The...