On occasion, asymmetry in the cell contour led to spurious pole positioning. The misplaced pole was then shifted ‘manually’ so as to maximize the symmetry of the curvature and fluorescence profiles. The measurement of kinematic variables such as curvature was performed at a fine spatial ...
37, an Hsp104 variant lacking the N domain (Hsp104ΔN) is functional and cooperates with Hsp70/40 in protein disaggregationin vitro(Fig.4a). Because the N domain adopts a stably folded conformation that does not make strong contacts with the D1 domain in the Hsp104 hexamer...