We also incorporated MS-based and enzyme-linked immunosorbent assay (ELISA) affinity measures of other high-value biomarker targets—such as Aβ and tau species—and different imaging and cognitive measures acquired in DIAN in the analysis to serve as benchmarks for the proteomic changes observed....
a, Design of a sequence library to assess the requirement and sufficiency of the TATA-box and CCAAT-box motifs in the core and proximal promoter region, respectively, for the BRD4-independent transcriptional activity with massive parallel reporter assay. For the loss of function approach (left) ...
Hierarchical clustering and principal components analysis (PCA) of the transcriptomic expression profiles both indicated that the replicates of each treatment were closely correlated in all cases, so no datasets needed to be discarded (Fig.3A, B). These analyses additionally revealed that samples were ...
carcinoma, as part of the Human Tumor Atlas Network14, we profiled single-nuclei transcriptomes (single-nucleus RNA sequencing (RNA-seq) (snRNA-seq)) and epigenomes (single-cell assay for transposase-accessible chromatin using sequencing (ATAC-seq) (scATAC-seq)) of healthy colon, polyps and ...
Structural analysis of mutants was performed by using Western blots to assay correct folding, which is required for processing to produce the mature p66 and p51 RT species. This analysis indicates that beta-strand 10 is a structurally important region. Combined analysis of these two assays ...
In the effort to develop cell‐based therapies to treat salivary gland dysfunction, many different populations of cells in the adult salivary glands have been proposed as stem cells. These cell populations vary, depending on the assay used, and are often non﹐verlapping, leading to the ...
In sum, a whole new field has emerged, devoted to the investigation of the molecular mechanisms, the cell biology, the physiological and pathological implications of contact sites. While it is now obvious that such appositions are central to the structure and function of any eukaryotic cell and...
From a clinical point of view, this raises the question of the detection of the STAT3–NFκB complex on tumor samples if this enhances or defines aggressive tumors in the absence of Y705 phosphorylation. Proximity liga- tion assay can theoretically be used to detect ...
These assays traditionally comprise capturing changes in mRNA stability or alternative splicing. More recently, a new assay to address the role of RBPs on mRNA translation was introduced termed ribosome profiling [47••]. It consists of an improvement of a ribosome footprinting approach [48] ...
The supernatant was used for the GSH assay following the manufacturer’s protocol. The absorbance of the solution at 405 nm was measured using a plate reader (Infinite M200; Tecan, Männedorf, Switzerland). Time course alteration of GSH was determined using cultured cells in a 96-well plate...