We validated the effect of BRAF and EGFR combination and MEK1/2 inhibitor drug resistance variants using arrayed proliferation assays and by analyzing cell signaling. Overall, we set out to validate 4 of 13 drug addiction and 3 of 36 canonical drug resistance gRNAs for dabrafenib and cetuximab,...
This allowed us to validate gRNAs at scale in an arrayed format. gRNA sequences are listed in Supplementary Tables 18 and 19. Senescence assays Oncogene-induced senescence was assessed by staining with the β-galactosidase staining kit 48 h after drug treatment in 96-well plates following ...
Board game with football theme - has interconnected dots arrayed in squares on surface to define movement paths for ball and player markersA game board which represents a football pitch, is divided up into a regular number of squares. Each square is also provided with the two diagonal lines. ...
(n = 10; four fetal and two adult samples of non-neoplastic cerebellum; two samples of Whole-Genome Amplified DNA (unmethylated control; two samples of M.SssI-treated DNA [100% methylated control]) were arrayed using the Illumina HumanMethylation450 BeadChip according to the manufacturer’s ...
In cyanobacteria, phycobilisomes are regularly arrayed on the surface of the photosynthetic membranes, and their role is to funnel light energy to the underlying photosystem II reaction center. A model has recently been proposed that ascribes to the so-called LCM, a central role in the ...