The env-like ORF begins with a putative splice acceptor site and encodes a protein with a predicted central transmembrane domain, similar to retroviral env genes. RNA of Athila elements was detected in an A. thaliana strain with decreased DNA methylation (ddm1). Additionally, a PC...
George Obiero2, Philip Bejon1,3, Jean Langhorne4, Abdirahman Abdi1 and Lynette Isabella Ochola‑Oyier1* Abstract Background Cumulative malaria parasite exposure in endemic regions often results in the acquisition of partial immunity and asymptomatic infections...
Intriguingly, a 32-nt RNA element at the 3′-end of transmissible gastroenteritis virus (TGEV), a porcine alphacoronavirus, has sequence homology, and similar secondary structure, with the human ceruloplasmin (Cp) GAIT (Gamma-interferon-Activated Inhibitor of Translation) RNA element present in a ...
metabolomics, tumor whole-exome sequencing, and transcriptome sequencing (Meta/liquid chromatography-mass spectrometry (LC–MS)/ WES/RNA-seq)]. Overall, 494 patients with RCC (n = 113), LCC (n = 193), or healthy controls (n = 188...
in four cancer cell lines using a guide RNA library predicted to install 32,476 variants in 11 cancer genes. We identify four functional classes of protein variants modulating drug sensitivity and use single-cell transcriptomics to reveal how these variants operate through distinct mechanisms, ...
Can messenger RNA code for multiple proteins? How are messenger RNA and transfer RNA similar? Do amino acids use messenger RNA? What enzyme transcribes DNA into messenger RNA? In which organelle is messenger RNA made? How is messenger RNA processed before leaving the eukaryotic cell? How does ...
George Obiero2, Philip Bejon1,3, Jean Langhorne4, Abdirahman Abdi1 and Lynette Isabella Ochola‑Oyier1* Abstract Background Cumulative malaria parasite exposure in endemic regions often results in the acquisition of partial immunity and asymptomatic infections...
There is a lack of well validated prognostic biomarkers in osteosarcoma, a rare, recalcitrant disease for which treatment standards have not changed in over 20 years. We performed microRNA sequencing in 74 frozen osteosarcoma biopsy samples, constituting
p63 proteins are structurally organized in domains, all isoforms having a DNA-binding domain (DBD) and an oligomerization domain (OD). While the TAp63 isoforms contain an N-terminus acidic transactivation domain (TA), the ΔNp63 ones maintain the transactivating capability due to the presence ...
DNA was extracted from cell pellets (Qiagen) and the gRNA cassette was PCR amplified (PCR1, 28 cycles, 3 µg per reaction) with several reactions in parallel to maintain the complexity of the library. After column purification of PCR1 products (Qiagen), PCR products were indexed by PCR...