Using full length genomic DNA next generation sequencing (NGS) for actual recipient and donor pairs, and sera known to contain DSA by single antigen beads (SAB), we developed a novel computer algorithm to define
Comparing the results of all approaches similar number of mismatches were observed.For both HLA class I and class II, we found that increasing numbers of AA mismatches resulted in a higher probability of DSA formation (as indicated in figure 1).This newly developed computer algorithm ...