Explain what will happen when reactants are added to a reversible reaction mixture at equilibrium. Write an equilibrium constant expression for the chemical reaction. SO_4^{2-} (aq) + H_2O (l) rightleftharpoons HSO_4^- (aq)+ OH^- (aq) Write an equilibrium constant ...
After column purification of PCR1 products (Qiagen), PCR products were indexed by PCR (PCR2, eight cycles, 0.2 ng per reaction). Libraries were sequenced on the HiSeq2500 (Illumina) using 19 bp SE sequencing on Rapid Run mode with a custom primer. All primers are listed in ...
Discuss the basic steps of how an enzyme catalyzes a reaction, using lactase as an example. What are enzymes, what do they do, how do they function? How do you assay an enzyme? A) How does an enzyme help a reaction proceed? B) How does heat, chemicals,...
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For cross-linking assays, PC12 cell extracts were incubated with the reversible homobifunctional cross-linker dithiobis(succinimidylpropionate) (DSP; Pierce), following the manufacturer’s instructions. Reaction was quenched with Tris buffer (pH 7.5), and cells were scraped in cold lysis buffer (PBS...
reversible (math.) invertible 不可逆 bù kě nì irreversible 逆光 nì guāng backlighting (lighting design) 逆向 nì xiàng backwards reverse direction 逆风 Trad. 逆風 nì fēng to go against the wind contrary wind a headwind 叛逆 pàn nì to rebel to revolt a rebel Example Usa...