Herein, we report that SIRT2, a potent lysine defatty-acylase, is upregulated by the transcription factor CREB3 under Golgi stress induced by Shigella infection. SIRT2 in turn removes the lysine fatty acylation introduced by Shigella virulence factor IcsB to enhance host innate immunity. SIRT2 ...
SIRT2-H187Y (HY), a previously reported dead deacetylase but weak defatty-acylase, was used as a control18. We co-overexpressed Flag-tagged IcsB substrate proteins, IcsB, and SIRT2 in HEK293T and examined the lysine fatty acylation on the substrate proteins by Alk14 labeling...
Development of a NanoBRET assay to validate dual inhibitors of Sirt2-mediated lysine deacetylation and defatty-acylation that block prostate cancer cell migrationdoi:10.26434/chemrxiv-2021-1r1dnAnja VogelmannMatthias SchiedelNathalie WoessnerManfred Jung...
(1983). Deacylation and uptake of the fatty acyl chain from enantiomeric dialkylacyl- glycerol by intact rat fat cells. Biochim. Bioptrys. Acfa, 752. 277-283.Benoit, V. and Boyer, J., 1983, Deacylation and uptake of the fatty acyl chain from enantiomeric dialkylacylglycerol by intact ...
(2013). Activation of the protein de- acetylase SIRT6 by long-chain fatty acids and widespread deacylation by mammalian sirtuins. J. Biol. Chem. 288, 31350-31356.Feldman JL, Baeza J, Denu JM. Activation of the protein deacetylase SIRT6 by long-chain fatty acids and widespread de...
The invention belongs to the filed of chemistry, and relates to application of thionyl chloride and alcohol as selective deacylation reagents for N-aryl fatty amide (I) or N-heterocyclic fatty amide (II or III) and a method thereof. The invention mainly comprises application of thionyl chloride...