100 mg of the crushed gum powder was suspended in 500 μL of plant extraction buffer (PEB) (100 mM NaCl; 10 mM EDTA; 200 mM Tris-HCl, pH 8.0; 0.05% (v/v) Tween-20; 1 x protease inhibitor cocktail (PIC); 0.1% SDS; 14 mM β -Mercapto-ethanol; 400 mM sucrose; and 2 mM ...