After centrifugation, the cell slurry was washed twice in MEME. Pre-warmed digestion buffer (HBSS, 0.05% collagenase IV, 0.5% fatty acid free BSA, 10 mM CaCl2) was added and the slurry placed in a shaking water bath again for 30 min at 37 °C. BSA was included in the digestion ...
13). These viral videos of Black death may have “deadening effects of over-exposure to images that were perhaps once meant to move us” (Juhasz 2016, para. 9), a concern consistent with how interest in a news event tends to wane (as conveyed by the “15 min of fame” expression)....
The Astor Estate: Let’s Get Ready to RumbleEvery new beginning, as they say, comes from some other beginning’s end, and from Brooke Astor’s death yesterday comes a new beginning that’s just getting warmed up: the fight over her fortune, estimated at $130 million. Astor’s son, Ant...