Briefly, viral particles were produced in HEK293- FT cells using the vector, VSVG, tat and DR8.2 plasmids. JVM-3 cells were transduced thrice with the viral media. Cells were grown for 72 h after the last transduction. The STAT3-C overexpression vector has an EGFP sequence as a ...
b Real-time PCR of FAO genes in ES2 cells co-cultured with CAFs expressing scrambled vector or shCOL11A1. The mRNA expression was normalized to RPL32. N = 3; y-axis, relative mRNA expression (fold change); error bars, SD; *p < 0.05; **p < 0.01. c Western blot of ...
we stably reconstitutedRipk1−/−cells with WT Ripk1, or with an empty control vector, using a doxycyclin-inducible system. Doxycycline treatment of reconstitutedRipk1−/−cells led to a level of Ripk1 expression comparable to the endogenous one (Figure 1d). In addition, in the absence ...
C Cell growth curve of Patu-8988t and PANC-1 cells endogenous overexpressing of vector or wild-type SENP3 or inactive mutant SENP3 via CCK8 assay. D Cell cycle analysis of Paut-8988t and PANC-1 cells endogenous overexpressing of vector or wild-type SENP3 or inactive mutant SENP3. E ...
Flowjo software (Tree Star Inc., Ashland, OR, USA) was used for analysis. Cells used for quantification expressed GFP along with constructs of interest to identify transfected/infected cells. The fraction of apoptotic cells was independently measured using YO-PRO-1 staining, according to the ...
EcoRV restriction enzyme was used to digest PCR product and pCCL-6HF vector [64], then TIE1 was subcloned into pCCL-6HF vector [64]. The pCCL-6HF-TIE1 construct was verified by DNA sequencing. 293T cells were transfected with a mix of 1/3 ΔHelper, 1/3 VsVg and 1/3 pCCL-6HF...
Viral particles to infect CCRF-CEM cells were produced in HEK 293 T cells. In total, 2 × 106cells were seeded in 10-cm plates. On the day of transfection, 3 μg of the plasmid pBABE.puro.hNICD or empty vector (pBABE), 3 μg enveloping vector VSVg, and 3 μg packaging...
As shown in Figure 2d, the cells transfected with procaspase-8 WT displayed much higher rate of the Δψ (21.225 ± 2.708%) than the control vector-transfected cells (4.085 ± 0.262%, p < 0.01), as well as the P10 mutations-transfected cells (3.475 ± 0.290% ...