After digestion, samples were filtered through metal mesh and centrifuged at 250 × g for 5 min at 4 °C. Then, the homogenate was resuspended in Trypsin-EDTA for further dissociation for 2 min at 37 °C and treated with 5 μg/ml DNase I (Sigma-Aldrich) for another 5 ...
Download File import instruction Abstract Selenoproteins have been shown to exhibit a variety of biological functions, including antioxidant functions, maintaining cellular redox balance, and heavy metal detoxification. UV irradiation-induced damage is partially mediated by increased oxygen radical production. ...
After incubation on ice for 30 min, the mixture was placed in a metal bath at 98 °C for 10 min to denaturate the proteins. Protein concentration and separation were accomplished by applying 80 V and 120 V voltages to NuPAGE® Novex® 4–12% Bis-Tris Protein Gels. Using...
5L), and when combined with Midiv-1, the number of γ-H2AX foci reached its peak within 2 h (Fig. 5M), which indicate no significance when irradiation alone or co-treated with Mdivi-1 inhibitors. These findings suggest that Parkin/BNIP3-mediated mitophagy can alleviate DNA damage and ...