It is often desirable to be able to specify a protein fold during design (such as triose-phosphate isomerase (TIM) barrels or cavity-containing NTF2s for small molecule binder and enzyme design32,33), and thus we further fine-tuned RFdiffusion to condition on secondary structure and/or fold...
It is often desirable to be able to specify a protein fold during design (such as triose-phosphate isomerase (TIM) barrels or cavity-containing NTF2s for small molecule binder and enzyme design32,33), and thus we further fine-tuned RFdiffusion to condition on secondary structure and/or fold...
Our structure-based de novo design strategy has high accuracy, as demonstrated by the cryoEM structures and achieves high selectivity by integrating both previously known binding motifs and introducing completely new interactions in a hyperstable small scaffold. As exemplified by our successful design ...
We reasoned that for the de novo design of allosterically coupled assemblies, the reductive nature of the MWC model could be an advantage: rather than having to model the complexities of side-chain-to-side-chain communication between distant sites, and the large number of corresponding micro-state...
In summary, we have successfully devised de novo Fc-based proteins that can modulate PlxnB1 function by means of lasso graft. The use of homodimeric and structurally rigid Fc as the grafting scaffold, with an ampule choice of the grafting loop sites, was critical in achieving specific functiona...
(2022) Isoform-specific inhibition of FGFR signaling achieved by a de-novo-designed mini-protein. Cell Rep 41(4):111545. 2022 Strukturbiologie/ Proteininteraktionen Zhou G et al. (2022) Co-alterations of circadian ...
(2022) Isoform-specific inhibition of FGFR signaling achieved by a de-novo-designed mini-protein. Cell Rep 41(4):111545. 2022 Structural biology/protein interactions Zhou G, et al. (2022) Co-alterations of circadian clo...
we chose to de novo design mini-protein binders as the starting point. These mini-protein binders were later developed into a broad neutralizing binder H3, which binds to major clinical TcdB variants (TcdB1-4) with sub-nanomolar affinities. Cryo-EM structures of mini-protein binder 43829 bound...
We reasoned that for the de novo design of allosterically coupled assemblies, the reductive nature of the MWC model could be an advantage: rather than having to model the complexities of side-chain-to-side-chain communication between distant sites, and the large number of corresponding micro-state...
The ability to design hybrid binders against non-repetitive sequences opens the door to the de novo design of binders against endogenous proteins. Intrinsically disordered regions have been very difficult to specifically target using other approaches, but are in principle good targets, because binding ...